Membrane protein, Nanodiscs, Protein structure, Thermodynamics, Protein-ligand interaction.
Stimulated by technological improvements, structural methodologies such as X-ray crystallography are increasingly contributing to accelerate the process of drug discovery.
We employ structure-based methodologies to validate hit compounds and design ligands of higher affinity.
Highly purified recombinant protein is subjected to crystallization trials using liquid handling systems.
Diffraction-quality crystals are generated in well-defined environmental conditions.
High-resolution structures of target proteins bound to ligands and hit compounds are determined by the soaking and/or the co-crystallization method.
We employ X-ray crystallography, and high-resolution biophysical techniques, to study proteins involved in human diseases such as cancer, infection, chronic pain, Parkinson’s disease, and AIDS (Figures 2-6).